Importance of the External Quality Control in Autoimmunity

Standardisation of autoantibody detection by indirect immunofluorescence (IIF) is based on identifying the staining pattern
of the reference material and titrating it under the same routine working conditions. On the other hand, autoantibody determinations performed by enzyme-linked immunosorbent assay (ELISA) may provide qualitative or quantitative results, depending on the manufacturer, expressed in arbitrary units. In both cases—IFI and ELISA — participation in external quality assessment programs represents the main tool on the path toward standardising autoantibody measurement.

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